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Quercetin improved mitochondrial membrane potential (MMP) and inhibited Rifampicin (RFP)-Induced apoptosis in HepaRG Cells. (A) CCK-8 experiment to determine the optimal Quercetin concentration for enhancing HepaRG cell viability ( n = 6). (B) CCK-8 assay to assess the protective effect of Quercetin (15 μm) on cell viability in RFP (25 μm)-treated HepaRG cells. Verteporfin (2 μm) was used to inhibit YAP ( n = 6). (C) Flow cytometry scatter plots and quantification of apoptosis rates of Annexin V/PI staining ( n = 3). (D) The mitochondrial membrane potential was assessed using tetramethylrhodamine ethyl ester (TMRE) and quantification of relative fluorescence intensity ( n = 3). Scale bars: 100 μm. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. One-way ANOVA followed by Tukey's post-hoc test was applied for multiple group comparisons for Type I error.

Journal: Frontiers in Medicine

Article Title: Quercetin alleviates rifampicin-induced hepatocyte injury by modulating the Hippo-YAP signaling pathway

doi: 10.3389/fmed.2025.1707248

Figure Lengend Snippet: Quercetin improved mitochondrial membrane potential (MMP) and inhibited Rifampicin (RFP)-Induced apoptosis in HepaRG Cells. (A) CCK-8 experiment to determine the optimal Quercetin concentration for enhancing HepaRG cell viability ( n = 6). (B) CCK-8 assay to assess the protective effect of Quercetin (15 μm) on cell viability in RFP (25 μm)-treated HepaRG cells. Verteporfin (2 μm) was used to inhibit YAP ( n = 6). (C) Flow cytometry scatter plots and quantification of apoptosis rates of Annexin V/PI staining ( n = 3). (D) The mitochondrial membrane potential was assessed using tetramethylrhodamine ethyl ester (TMRE) and quantification of relative fluorescence intensity ( n = 3). Scale bars: 100 μm. Data are mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001. One-way ANOVA followed by Tukey's post-hoc test was applied for multiple group comparisons for Type I error.

Article Snippet: Mitochondrial membrane potential (MMP) was evaluated using tetramethylrhodamine ethyl ester (TMRE) staining (C2001S, Beyotime, China).

Techniques: Membrane, CCK-8 Assay, Concentration Assay, Flow Cytometry, Staining, Fluorescence